Multiplex Fluorescent In Situ Hybridization Protocol for Drosophila Embryos

This site presents a more formal version of the information presented at this link.

Introduction
Embryo Gallery
Protocol Overview
Embryo Fixation
Embryo Treatment Before Hybridization
Hybridization and Making RNA Probes
Post-Hybridization Fluorescent Detection
Presentation of Embryos for Fluorescence Microscopy
Simultaneous Detection of mRNA and Protein
Alternative Methods for Fluorescent Detection
Nuclear Dots and Virtual Colors
Conclusions and Future Problems
Reagents and Recipes
Links and References
Questions

Prepared by Dave Kosman (Ethan Bier and Bill McGinnis labs, UC San Diego)
Protocols developed in collaboration with Mieko Mizutani and Greg Cox
Project supported by NSF IBN0120728